In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Figure 3. Molecular Devices. SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. Tunable wavelength selection / monochromator. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Cables. SpectraMax® M5e. Supplier: Molecular Devices. The SpectraMax M2 and M2e are versatile and reliable. Examples: “Press carefully to engage,” “ Do not. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 303 unable to cal dark current. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 00. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. Fill a clean plate with water. The advantage of using labeled probes is detection specificity. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. You should get comparable results from top and bottom reads using EC50's to compare reads. 7 1. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. When a FlexStation® 3 Multi-Mode Microplate Reader. Pick the best speed, sensitivity, and price for your needs. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Most papers they cultured bacteria to OD600>0. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Description. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Sale price $16,495. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. 5 Dual-luciferase reporter assay. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The acceptance. AlphaScreen. Ltd. A: Microplate Preparation. used. 2. Sale price $18,499. free tracer decreases, resulting in. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. begin reading microplates with seven mouse clicks. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). . The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Retrieving Each. Five preferred detection modes. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 2. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Please refer to the Obsolete Microplate Reader Models for more information. Has anyone had problems with the built-in computer of the Spectramax ID3. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. The CDWIA was performed on each. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The. A: Microplate Preparation. Ltd. Here the delta mP was 336, and Z’ factor was 0. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The assay applies fluorescence polarization to quantify Fc-containing IgG. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 1. 58% for the detection module and 2. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. SpectraMax® Aquamax. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Table 1. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. M3-M5e_manual. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Shop now. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. 1–0. 3. Softmax Pro GxP Software 6. 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. STAKMAX. The SpectraMax M5e Multi. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. Expanded wavelength coverage. View the Molecular Devices website. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Make sure the microplate is flat against. 313 reference gain check fail. SpectraMax iD5 Multi-Mode Microplate Reader. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The percentage of viable cells for each treatment group was calculated by adjusting the untreated control group to 100%. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. edu) to be added as a trained user in iLab. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. All associated StakMax® stackers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. e. Fluorescence intensity was detected by. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. Figure 4: Transcreener ADP. The CellTiter-Glo assay from Promega uses luciferase. E Models Only) 69. Cuvette port functionality can also be controlled using SoftMax Pro Software. For the Live/Dead assay, they were treated. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 1 2. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Here the delta mP was 336, and Z’ factor was 0. Selection of Water Constant in PathCheck settings. 4 Max StDev 1. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. All associated StakMax® stackers. 1step assay with SpectraMax microplate readers. 63 1. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. 6 0. Parallel negative control assays had primary, secondary, or both antibodies omitted. edu) to be added as a trained user in iLab. The chamber contains springs that position the cuvette in the proper alignment for a read. 0 out of 5 $ 3,799. view more . When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax M5e Multi-Mode Microplate Reader. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Devices’ StakMax® Microplate Stacker in a matter of minutes and . SpectraMax 250 User. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax® ABS Plus. Figure 1. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. SpectraMax® ABS. SpectraMax® Aquamax. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. View the Molecular Devices website. M3-M5e_manual. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Keywords. • Tel. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. The dual monochromator optics allow the widest. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 3 Version 7. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M5 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Cuvette port functionality can also be controlled using SoftMax Pro Software. 314 low lamp level warning. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Bookings are free, but you will need to use iLab to book. Figure 4: Transcreener ADP. Table of Contents. 1** Version 7. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. 3 mg. 1. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 7. Three independent experiments were performed. The chamber contains springs that position the cuvette in the proper alignment for a read. Both readers feature an 8-channel read head for fast reads and. microplate reader. The dual monochromator optics allow the widest range of applications to be. As. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. 1. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. RAW 264. 306 plate air cal. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). The samples were boiled for 5 min with 1 ×. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Bookings are free, but you will need to use iLab to book. SpectraMax® M4. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Molecular Devices. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). 0 out of 5 $ 24,695. book Page 7 Wednesday, July 7, 2010 2:09 PM. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The assay window spanned four orders of magnitude. 2. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Figure 1. No accessories are needed and SoftMax® Pro Software. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. com A. We would like to show you a description here but the site won’t allow us. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. For the Live/Dead assay, they were treated. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. com. All assays were performed in at least three individual experiments, each comprising no. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 72 MB. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Molecular Devices Spectramax M5 Microplate Reader. TNF standard curve. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. D. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 04 to 3. p. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. When a feature or capability applies to only certain readers, this exception is noted. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. Designed to provide the simplicity, flexibility and power required for advanced data analysis. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Shop now. Incubation. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. 2. Methods Preparation of reagents and standards. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 54 1. Italic and boldface type are used for emphasis. Between steps, reactions were rinsed 3 times with TBS + 0. 7°C and the bottom to 36. Label-free detection. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. keeping with best practices, reader performance should. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. 6-384 (96- and 384-well with pipetting) 6-384. At the end of this period, cells were exposed to FA (1 mM) for 1 h. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Equipped with scanning, shaking and kinetics modules. TNF standard curve. Microplate formats. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. l. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Molecular Devices Corporation. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The spectrophotometric. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. RAW 264. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Add to Cart. Analyst, M5, Flex 3, and SpectraMax L readers work differently. book Page 7 Wednesday, July 7, 2010 2:09 PM. Nontreated cells in DMEM medium were used as a control. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 96-1536. Microplate Readers Biotek Epoch Microplate. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Table of Contents. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The spectrophotometric. The cells were then treated to induce apoptosis. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 2. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. We use Spectramax M5 microplate reader in our lab. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. FlexStation™ II. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. 1. 26 1. 43 Max %CV 1. When a feature or capability applies to only certain readers, this exception is noted. Category 2 and 3 microplate readers use gradient heating as well. Bottom-reading is usually recommended for cell-based assay with adherent cells. 81). Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Introduction. Wash Buffer Concentrate was diluted 10-fold. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. However, when a specific probe is not available, or it causes off-target effects on the. The drawing shows a standard microplate of 15 mm height covered with a lid. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). "With this complete portfolio of multi-mode configurations, we. Flexible for 6-well to 384-well microplates and cuvettes. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 04 to 3. The SpectraMax M2 and M2e are versatile and reliable. Microplate Reader Documentation Version No. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. 0 out of 5 $ 16,499. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Filterless readers provide consistent results with steady temperature regulation. Wide range of applications. Molecular Devices Spectramax M5 Microplate Reader. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Add to Cart. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. When a. Thank you!.